000 02710nab a2200373 c 4500
001 koha000902710
005 20221102170015.0
007 cr |
008 221031|2021 sz s a eng d
024 7 _a10.3390/plants10091831
_2doi
035 _akoha000902710
040 _aRU-ToGU
_brus
_cRU-ToGU
245 1 0 _aApplying biotechnology in the propagation and further selection of vaccinium uliginosum × (V. corymbosum × V. angustifolium) hybrids
_cA. A. Erst, A. B. Gorbunov, S. V. Asbaganov [et al.]
336 _aТекст
337 _aэлектронный
504 _aБиблиогр.: 28 назв.
520 3 _aThe most serious problem of intergeneric and interspecific hybridization is related to overcoming the reproductive isolation of different species. We assessed the efficiency of reproduction under in vitro conditions and the ex vitro growth capacity of interspecific hybrids of Vaccinium uliginosum (V. corymbosum V. angustifolium). The percentage of seed germination in in vitro culture was 88% for V. uliginosum, form No. 8 (V. corymbosum V. angustifolium), SC5-8, while it was 42% for V. uliginosum, form No. 8 (V. corymbosum V. angustifolium), ‘Northcountry’. The analysis of mean value showed that the multiplication rate increased and the shoot height decreased as the 2-isopentenyl adenine (2iP) concentration was increased in the nutrient medium of the studied hybrids. The maximum rate was achieved using 15 M 2iP. A detailed analysis of the hybrids indicated that the hybrid variant reliably affected growth and development indicators. Inter simple sequence repeat analysis demonstrated that all analyzed hybrids inherited DNA fragments of the parent plants in various combinations, confirming their hybrid nature. Thus, the use of in vitro methods for the propagation and further selection of genotypes is demonstrated as being an effective approach for developing interspecific hybrids of V. uliginosum (V. corymbosum V. angustifolium).
653 _aмежвидовые гибриды
653 _aягодные культуры
653 _aкультура in vitro
653 _aISSR-анализ
655 4 _aстатьи в журналах
_9817065
700 1 _aErst, Anna A.
_9491900
700 1 _aGorbunov, Aleksey B.
_9491901
700 1 _aAsbaganov, Sergey V.
_9817066
700 1 _aTomoshevich, Maria A.
_9816972
700 1 _aBanaev, Evgeny V.
_9817067
700 1 _aErst, Andrey S.
_9248121
773 0 _tPlants
_d2021
_gVol. 10, № 9. P. 1831 (1-11)
_x2223-7747
852 4 _aRU-ToGU
856 4 _uhttp://vital.lib.tsu.ru/vital/access/manager/Repository/koha:000902710
908 _aстатья
999 _c902710