| 000 | 03370nab a2200433 c 4500 | ||
|---|---|---|---|
| 001 | koha000893822 | ||
| 005 | 20220506173925.0 | ||
| 007 | cr | | ||
| 008 | 220505|2021 xxu s a eng d | ||
| 024 | 7 |
_a10.1007/s10815-020-02003-1 _2doi |
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| 035 | _akoha000893822 | ||
| 040 |
_aRU-ToGU _brus _cRU-ToGU |
||
| 245 | 1 | 0 |
_aLINE-1 retrotransposon methylation in chorionic villi of first trimester miscarriages with aneuploidy _cS. A. Vasilyev, E. N. Tolmacheva, O. Yu. Vasilyeva [et al.] |
| 336 | _aТекст | ||
| 337 | _aэлектронный | ||
| 504 | _aБиблиогр.: 72 назв. | ||
| 520 | 3 | _aPurpose High frequency of aneuploidy in meiosis and cleavage stage coincides with waves of epigenetic genome reprogramming that may indicate a possible association between epigenetic mechanisms and aneuploidy occurrence. This study aimed to assess the methylation level of the long interspersed repeat element 1 (LINE-1) retrotransposon in chorionic villi of first trimester miscarriages with a normal karyotype and aneuploidy. Methods The methylation level was assessed at 19 LINE-1 promoter CpG sites in chorionic villi of 141 miscarriages with trisomy of chromosomes 2, 6, 8-10, 13-15, 16, 18, 20-22, and monosomy X using massive parallel sequencing. Results The LINE-1 methylation level was elevated statistically significant in chorionic villi of miscarriages with both trisomy (45.2 +/- 4.3%) and monosomy X (46.9 +/- 4.2%) compared with that in induced abortions (40.0 +/- 2.4%) (p < 0.00001). The LINE-1 methylation levels were specific for miscarriages with different aneuploidies and significantly increased in miscarriages with trisomies 8, 14, and 18 and monosomy X (p < 0.05). The LINE-1 methylation level increased with gestational age both for group of miscarriages regardless of karyotype (R = 0.21, p = 0.012) and specifically for miscarriages with trisomy 16 (R = 0.48, p = 0.007). LINE-1 methylation decreased with maternal age in miscarriages with a normal karyotype (R = - 0.31, p = 0.029) and with trisomy 21 (R = - 0.64, p = 0.024) and increased with paternal age for miscarriages with trisomy 16 (R = 0.38, p = 0.048) and monosomy X (R = 0.73, p = 0.003). Conclusion Our results indicate that the pathogenic effects of aneuploidy in human embryogenesis can be supplemented with significant epigenetic changes in the repetitive sequences. | |
| 653 | _aретротранспозоны | ||
| 653 | _aметилирование ДНК | ||
| 653 | _aанеуплоидия | ||
| 655 | 4 |
_aстатьи в журналах _9803860 |
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| 700 | 1 |
_aVasilyev, Stanislav A. _9103013 |
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| 700 | 1 |
_aTolmacheva, Ekaterina N. _9803861 |
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| 700 | 1 |
_aVasilyeva, Oksana Yu. _9803863 |
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| 700 | 1 |
_aMarkov, Anton V. _999149 |
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| 700 | 1 |
_aZhigalina, Daria I. _9103725 |
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| 700 | 1 |
_aZatula, Lada A. _9803864 |
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| 700 | 1 |
_aLee, Vasilissa A. _9803865 |
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| 700 | 1 |
_aSerdyukova, Ekaterina S. _9803866 |
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| 700 | 1 |
_aSazhenova, Elena A. _9803867 |
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| 700 | 1 |
_aNikitina, Tatyana V. _9803868 |
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| 700 | 1 |
_aKashevarova, Anna A. _9106156 |
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| 700 | 1 |
_aLebedev, Igor N. _d1974- _9106151 |
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| 773 | 0 |
_tJournal of assisted reproduction and genetics _d2021 _gVol. 38, № 1. P. 139-149 _x1058-0468 |
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| 852 | 4 | _aRU-ToGU | |
| 856 | 4 | _uhttp://vital.lib.tsu.ru/vital/access/manager/Repository/koha:000893822 | |
| 908 | _aстатья | ||
| 999 | _c893822 | ||