| 000 | 03106nab a2200385 c 4500 | ||
|---|---|---|---|
| 001 | vtls000707359 | ||
| 003 | RU-ToGU | ||
| 005 | 20230214175844.0 | ||
| 007 | cr | | ||
| 008 | 200302|2019 sz s a eng dd | ||
| 024 | 7 |
_a10.33594/000000162 _2doi |
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| 035 | _ato000707359 | ||
| 040 |
_aRU-ToGU _brus _cRU-ToGU |
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| 245 | 1 | 0 |
_aElevation of intracellular Na+ contributes to expression of early response genes triggered by endothelial cell shrinkage _cA. A. Shiyan, S. V. Sidorenko, D. Fedorov [et al.] |
| 504 | _aБиблиогр.: 53 назв. | ||
| 520 | 3 | _aBACKGROUND/AIMS: Prolonged hyperosmotic shrinkage evokes expression of osmoprotective genes via nuclear factor NFAT5-mediated pathway and activates Na+ influx via hypertonicity-induced cation channels (HICC). In human umbilical vein endothelial cells (HUVEC) elevation of intracellular sodium concentration ([Na+]i) triggers transcription of dozens of early response genes (ERG). This study examined the role of monovalent cations in the expression of Na+i-sensitive ERGs in iso- and hyperosmotically shrunken HUVEC. METHODS: Cell volume was measured by 3D reconstruction of cell shape and as 14C-urea available space. Intracellular Na+ and K+ content was measured by flame atomic absorption spectrometry. ERG transcription was estimated by RT-PCR. RESULTS: Elevation of medium osmolality by 150 mM mannitol or cell transfer from hypo- to isosmotic medium decreased cell volume by 40-50%. Hyperosmotic medium increased [Na+]i by 2-fold whereas isosmotic shrinkage had no impact on this parameter. Hyperosmotic but not isosmotic shrinkage increased up-to 5-fold the content of EGR1, FOS, ATF3, ZFP36 and JUN mRNAs. Expression of these ERGs triggered by hyperosmotic shrinkage and Na+,K+-ATPase inhibition by 0.1 µM ouabain exhibited positive correlation (R2=0.9383, p=0.0005). Isosmotic substitution of NaCl by N-methyl-D-glucamine abolished an increment of [Na+]i and ERG expression triggered by mannitol addition. CONCLUSION: Augmented expression of ERGs in hyperosmotically shrunken HUVEC is mediated by elevation of [Na+]i. | |
| 653 | _aэндотелий | ||
| 653 | _aгены раннего ответа | ||
| 653 | _aвнутриклеточная концентрация натрия | ||
| 653 | _aодновалентные катионы | ||
| 655 | 4 |
_aстатьи в журналах _9745982 |
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| 700 | 1 |
_aSidorenko, Svetlana V. _9474351 |
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| 700 | 1 |
_aFedorov, Dmitry _9501256 |
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| 700 | 1 |
_aKlimanova, Elizaveta A. _9480046 |
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| 700 | 1 |
_aSmolyaninova, Larisa V. _9567804 |
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| 700 | 1 |
_aKapilevich, Leonid V. _d1963- _9100191 |
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| 700 | 1 |
_aGrygorczyk, Ryszard _9345910 |
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| 700 | 1 |
_aOrlov, Sergei N. _999633 |
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| 700 | 1 |
_aShiyan, Alexandra A. _9501258 |
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| 773 | 0 |
_tCellular physiology and biochemistry _d2019 _gVol. 53, № 4. P. 638-647 _x1015-8987 |
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| 852 | 4 | _aRU-ToGU | |
| 856 | 4 | _uhttp://vital.lib.tsu.ru/vital/access/manager/Repository/vtls:000707359 | |
| 908 | _aстатья | ||
| 999 | _c464466 | ||