| 000 | 03271nab a2200373 c 4500 | ||
|---|---|---|---|
| 001 | vtls000532302 | ||
| 003 | RU-ToGU | ||
| 005 | 20230214175848.0 | ||
| 007 | cr | | ||
| 008 | 181202|2015 fr s a eng dd | ||
| 024 | 7 |
_a10.1016/j.bbamem.2015.07.005 _2doi |
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| 035 | _ato000532302 | ||
| 040 |
_aRU-ToGU _brus _cRU-ToGU |
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| 245 | 1 | 0 |
_aRole of cytoskeleton network in anisosmotic volume changes of intact and permeabilized A549 cells _cA. Platonova, O. Ponomarchuk, F. Boudreault [et.al.] |
| 504 | _aБиблиогр.: 39 назв. | ||
| 520 | 3 | _aRecently we found that cytoplasm of permeabilized mammalian cells behaves as a hydrogel displaying intrinsic osmosensitivity. This study examined the role of microfilaments and microtubules in the regulation of hydrogel osmosensitivity, volume-sensitive ion transporters, and their contribution to volume modulation of intact cells. We found that intact and digitonin-permeabilized A549 cells displayed similar rate of shrinkage triggered by hyperosmotic medium. It was significantly slowed-down in both cell preparations after disruption of actin microfilaments by cytochalasin B, suggesting that rapid water release by intact cytoplasmic hydrogel contributes to hyperosmotic shrinkage. In hyposmotic swelling experiments, disruption of microtubules by vinblastine attenuated the maximal amplitude of swelling in intact cells and completely abolished it in permeabilized cells. The swelling of intact cells also triggered ~ 10-fold elevation of furosemide-resistant 86Rb+ (K+) permeability and the regulatory volume decrease (RVD), both of which were abolished by Ba2 +. Interestingly, RVD and K+ permeability remained unaffected in cytocholasin/vinblastine treated cells demonstrating that cytoskeleton disruption has no direct impact on Ba2 +-sensitive K+-channels involved in RVD. Our results show, for the first time, that the cytoskeleton network contributes directly to passive cell volume adjustments in anisosmotic media via the modulation of the water retained by the cytoplasmic hydrogel. | |
| 653 | _aцитоскелеты | ||
| 653 | _aмикрофиламенты | ||
| 653 | _aгидрогели | ||
| 655 | 4 |
_aстатьи в журналах _9681159 |
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| 700 | 1 |
_aPlatonova, Alexandra _9345907 |
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| 700 | 1 |
_aBoudreault, Francis _9345908 |
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| 700 | 1 |
_aKapilevich, Leonid V. _d1963- _9100191 |
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| 700 | 1 |
_aMaksimov, Georgy V. _9345909 |
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| 700 | 1 |
_aGrygorczyk, Ryszard _9345910 |
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| 700 | 1 |
_aOrlov, Sergei N. _999633 |
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| 700 | 1 |
_aPonomarchuk, Olga O _9345911 |
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| 710 | 2 |
_aТомский государственный университет _bФакультет физической культуры _bКафедра спортивно-оздоровительного туризма, спортивной физиологии и медицины _986145 |
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| 773 | 0 |
_tBiochimica et Biophysica Acta (BBA) – Biomembranes _d2015 _gVol. 1848, № 10 (A). P. 2337-2343 _x0005-2736 |
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| 852 | 4 | _aRU-ToGU | |
| 856 | 7 | _uhttp://vital.lib.tsu.ru/vital/access/manager/Repository/vtls:000532302 | |
| 908 | _aстатья | ||
| 999 | _c259744 | ||