TY  - SER
AU  - Zhuo,Guan-Yu
AU  - Spandana,K.U.
AU  - Sindhoora,K.M.
AU  - Kistenev,Yury V.
AU  - Kao,Fu-Jen
AU  - Nikolaev,Viktor V.
AU  - Zuhayri,Hala
AU  - Krivova,Natalya A.
AU  - Mazumder,Nirmal
TI  - Label-free multimodal nonlinear optical microscopy for biomedical applications
KW  - биомедицинские приложения
KW  - нелинейная оптическая микроскопия
KW  - неинвазивная молекулярная визуализация
KW  - статьи в журналах
N1  - Библиогр.: с. 214901-13-214901-14; Ограниченный доступ
N2  - This paper addresses the application of multimodal nonlinear optical (MNLO) microscopy to clinical research within the context of label-free non-invasive molecular imaging. Here, a compact MNLO microscope based on a laser scanning microscope, a femtosecond laser, a time-correlated single-photon counting system, and a photonic crystal fiber are introduced for biomedical applications. By integrating two-photon fluorescence, two-photon fluorescence lifetime imaging, second-harmonic generation, and coherent anti-Stokes Raman scattering microscopy, the proposed scheme provides profound insights into the physicochemical properties related to 3D molecular orientation distribution, inter- and intra-molecular interactions, and disease progression in biological systems and organs. The high peak power and the low average intensity of near-infrared laser pulses allow for deep-penetration imaging without compromising sample vitality. Linking nonlinear optical phenomena with time/spectral/polarization-resolved imaging also makes it possible to obtain multidimensional information to address complex biomedical questions
UR  - http://vital.lib.tsu.ru/vital/access/manager/Repository/koha:000892189
ER  -