Влияние нокаута генов ADAMTS1, RBFOX2 и THBS1 на транскрипционную регуляцию и клональную выживаемость клеток в модельной системе in vitro А. А. Мурашкина, Р. Р. Савченко
Material type:
ArticleOther title: Effects of knockout ov ADAMTS1, RBFOX2 and WHSC1 genes on transcription regulation and clonogenic survival in the model system in vitro [Parallel title]Subject(s): гены | радиочувствительность | онкологические заболевания | двунитевые разрывы ДНК | нокаут геновGenre/Form: статьи в сборниках Online resources: Click here to access online
In:
Перспективы развития фундаментальных наук. Т. 4 : сборник научных трудов XV Международной конференции студентов, аспирантов и молодых ученых, 24-27 апреля 2018 г Т. 4 : Биология и фундаментальная медицина. С. 108-109Abstract: In this study, we evaluated the expression level and clonogenic survival of cell lines with a mutation of the ADAMTSl, RBFOX2, and THBSl genes generated by CRISPR/Cas9 technology. The gene expression profile was analyzed by expression microarray. It was found that knockout of all analyzed genes leads to a decrease in their own expression. In addition, the knockout of these genes leads to significant changes in the expression of other genes in the cell lines. Clonogenic survival of the lines was decreased in the cell lines with knockout of ADAMTSl and RBFOX2 genes after exposure to 2 Gy of y-rays.
Библиогр.: 3 назв.
In this study, we evaluated the expression level and clonogenic survival of cell lines with a mutation of the ADAMTSl, RBFOX2, and THBSl genes generated by CRISPR/Cas9 technology. The gene expression profile was analyzed by expression microarray. It was found that knockout of all analyzed genes leads to a decrease in their own expression. In addition, the knockout of these genes leads to significant changes in the expression of other genes in the cell lines. Clonogenic survival of the lines was decreased in the cell lines with knockout of ADAMTSl and RBFOX2 genes after exposure to 2 Gy of y-rays.
There are no comments on this title.