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Genetic Transformation Systems in Fungi, Volume 2 electronic resource edited by Marco A. van den Berg, Karunakaran Maruthachalam.

Contributor(s): van den Berg, Marco A [editor.] | Maruthachalam, Karunakaran [editor.] | SpringerLink (Online service)Material type: TextTextSeries: Fungal BiologyPublication details: Cham : Springer International Publishing : Imprint: Springer, 2015Description: XIV, 270 p. 38 illus., 26 illus. in color. online resourceContent type: text Media type: computer Carrier type: online resourceISBN: 9783319105031Subject(s): Life Sciences | Systems Biology | Developmental biology | Plant genetics | Plant Pathology | Plant breeding | Life Sciences | Developmental Biology | Plant Genetics & Genomics | Systems Biology | Plant Breeding/Biotechnology | Plant PathologyDDC classification: 571.8 LOC classification: QH491Online resources: Click here to access online
Contents:
Anastomosis and Heterokaryon Formation -- Induction of the Sexual Cycle in Filamentous Ascomycetes -- What Have We Learned by Doing Transformations in Neurospora tetrasperma? -- Repeat-induced Point Mutation: A Fungal-specific, Endogenous Mutagenesis Process -- Calculating RIP Mutation in Fungal Genomes Using RIPCAL -- Fungal Transposable Elements -- In vivo Targeted Mutagenesis in Yeast using TaGTEAM -- RNA Silencing in Filamentous Fungi: from Basic to Applications -- RNAi-mediated Gene Silencing in the Beta-lactam Producers Fungi Penicillium chrysogenum and Acremonium chrysogenum -- Controlling Fungal Gene Expression Using the Doxycycline-Dependent Tet-ON System in Aspergillus fumigates -- Expanding the Repertoire of Selectable Markers for Aspergillus Transformation -- Arginase (agaA) as a Fungal Transformation Marker -- Transformation of Ascomycetous Fungi using Autonomously Replicating Vectors -- A Recyclable and Bidirectionally Selectable Marker System for Transformation of Trichoderma -- Split Marker-Mediated Transformation and Targeted Gene Disruption in Filamentous Fungi -- Integrated Automation for Continuous High-Throughput Synthetic Chromosome Assembly and Transformation to Identify Improved Yeast Strains for Industrial Production of Biofuels and Bio-based Chemicals -- Imaging Flow Cytometry and High-throughput Microscopy for Automated Macroscopic Morphological Analysis of Filamentous Fungi -- Yeast Cell Electroporation in Droplet-Based Microfluidic Chip -- Identification of T-DNA Integration Sites: TAIL-PCR and Sequence Analysis -- Genetic and Genomic Manipulation in Aspergillus niger -- Genetic Manipulation of Meyerozyma guilliermondii.
In: Springer eBooksSummary: Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. While Volume 1 provided its readers with an in-depth understanding of how the transformation of DNA is used to understand the genetic basis behind these fungal traits, Volume 2 of this text describes the transformation associated methods, tools, and many relevant elements available to the modern fungal researcher. With ample illustrative examples and protocols, Genetic Transformation Systems in Fungi, Volume 2 is meant not only as a reference guide for the experienced researcher, but also as an a learning tool for the emerging scientist.
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Anastomosis and Heterokaryon Formation -- Induction of the Sexual Cycle in Filamentous Ascomycetes -- What Have We Learned by Doing Transformations in Neurospora tetrasperma? -- Repeat-induced Point Mutation: A Fungal-specific, Endogenous Mutagenesis Process -- Calculating RIP Mutation in Fungal Genomes Using RIPCAL -- Fungal Transposable Elements -- In vivo Targeted Mutagenesis in Yeast using TaGTEAM -- RNA Silencing in Filamentous Fungi: from Basic to Applications -- RNAi-mediated Gene Silencing in the Beta-lactam Producers Fungi Penicillium chrysogenum and Acremonium chrysogenum -- Controlling Fungal Gene Expression Using the Doxycycline-Dependent Tet-ON System in Aspergillus fumigates -- Expanding the Repertoire of Selectable Markers for Aspergillus Transformation -- Arginase (agaA) as a Fungal Transformation Marker -- Transformation of Ascomycetous Fungi using Autonomously Replicating Vectors -- A Recyclable and Bidirectionally Selectable Marker System for Transformation of Trichoderma -- Split Marker-Mediated Transformation and Targeted Gene Disruption in Filamentous Fungi -- Integrated Automation for Continuous High-Throughput Synthetic Chromosome Assembly and Transformation to Identify Improved Yeast Strains for Industrial Production of Biofuels and Bio-based Chemicals -- Imaging Flow Cytometry and High-throughput Microscopy for Automated Macroscopic Morphological Analysis of Filamentous Fungi -- Yeast Cell Electroporation in Droplet-Based Microfluidic Chip -- Identification of T-DNA Integration Sites: TAIL-PCR and Sequence Analysis -- Genetic and Genomic Manipulation in Aspergillus niger -- Genetic Manipulation of Meyerozyma guilliermondii.

Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. While Volume 1 provided its readers with an in-depth understanding of how the transformation of DNA is used to understand the genetic basis behind these fungal traits, Volume 2 of this text describes the transformation associated methods, tools, and many relevant elements available to the modern fungal researcher. With ample illustrative examples and protocols, Genetic Transformation Systems in Fungi, Volume 2 is meant not only as a reference guide for the experienced researcher, but also as an a learning tool for the emerging scientist.

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