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Derivative Spectrophotometry and PAM-Fluorescence in Comparative Biochemistry electronic resource by Vladimir S. Saakov, Alexander I. Krivchenko, Eugene V. Rozengart, Irina G. Danilova.

By: Saakov, Vladimir S [author.]Contributor(s): Krivchenko, Alexander I [author.] | Rozengart, Eugene V [author.] | Danilova, Irina G [author.] | SpringerLink (Online service)Material type: TextTextPublication details: Cham : Springer International Publishing : Imprint: Springer, 2015Edition: 1st ed. 2015Description: XIII, 611 p. 293 illus., 1 illus. in color. online resourceContent type: text Media type: computer Carrier type: online resourceISBN: 9783319115962Subject(s): Life Sciences | Biochemistry | Bioinformatics | Computational biology | Biophysics | Biological physics | Life Sciences | Computer Appl. in Life Sciences | Biophysics and Biological Physics | Biochemistry, generalDDC classification: 570.285 LOC classification: QH324.2-324.25Online resources: Click here to access online
Contents:
Introduction. Development of the methodological base, disputes, conclusions -- Successes of the pulse amplitude modulated fluorescence application -- Methodological approaches in our experimental work -- Application of Derivative Spectrophotometry in Comparative Biochemical Studies -- The Range of DHSO Application in Experiments with Pigments of Plants and Animals -- Conclusion.
In: Springer eBooksSummary: This book presents various examples of how advanced fluorescence and spectroscopic analytical methods can be used in combination with computer data processing to address different biochemical questions. The main focus is on evolutionary biochemistry and the description of biochemical and metabolic issues; specifically, the use of pulse amplitude modulated fluorescence (PAM) for the functional analysis of the cellular state, as well as results obtained by means of the derivative spectroscopy method characterizing structural reorganization of a cell under the influence of external factors, are discussed. The topics presented here will be of interest to biologists, geneticists, biophysicists and biochemists, as well as experts in analytical chemistry, pharmaceutical chemistry and radiochemistry and radioactivation studies with protonen and alpha-particles. It also offers a valuable resource for advanced undergraduate and graduate students in biological, physical and chemical disciplines whose work involves derivative spectrophotometry and PAM-fluorescence.
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Introduction. Development of the methodological base, disputes, conclusions -- Successes of the pulse amplitude modulated fluorescence application -- Methodological approaches in our experimental work -- Application of Derivative Spectrophotometry in Comparative Biochemical Studies -- The Range of DHSO Application in Experiments with Pigments of Plants and Animals -- Conclusion.

This book presents various examples of how advanced fluorescence and spectroscopic analytical methods can be used in combination with computer data processing to address different biochemical questions. The main focus is on evolutionary biochemistry and the description of biochemical and metabolic issues; specifically, the use of pulse amplitude modulated fluorescence (PAM) for the functional analysis of the cellular state, as well as results obtained by means of the derivative spectroscopy method characterizing structural reorganization of a cell under the influence of external factors, are discussed. The topics presented here will be of interest to biologists, geneticists, biophysicists and biochemists, as well as experts in analytical chemistry, pharmaceutical chemistry and radiochemistry and radioactivation studies with protonen and alpha-particles. It also offers a valuable resource for advanced undergraduate and graduate students in biological, physical and chemical disciplines whose work involves derivative spectrophotometry and PAM-fluorescence.

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